Engineered hosts with exogenous ligninase and uses thereof

DWPI Title: Engineered Bacillus host for treating lignin or derivative, comprises one or more exogenous nucleic acid sequences, where exogenous nucleic acid sequence encodes fungal laccase and fungal peroxidase, where peroxidase is versatile peroxidase, and host is Bacillus subtilis bacterium
Abstract: The present invention relates to methods and engineered microbial hosts useful for treating lignin or a derivative thereof. In some embodiments, the host has one or more exogenous nucleic acid sequences that encode a ligninase (e.g., a laccase and/or a peroxidase).
Use: Engineered Bacillus host for treating lignin or derivative.
Advantage: Engineered Bacillus host treats lignin, which is a renewable resource that requires depolymerization to release high value chemical intermediates.
Novelty: Engineered Bacillus host comprises one or more exogenous nucleic acid sequences, where exogenous nucleic acid sequence encodes fungal laccase and fungal peroxidase. The laccase comprises an amino acid sequence having 90% sequence identity sequence selected from sequences comprising 496-497 amino acid residues of (SEQ ID NOs: 1-4, 10, and 11), given in the specification. The peroxidase comprises an amino acid sequence having 80% sequence selected from sequences comprising 331 amino acid residues of (SEQ ID NOs: 20-25), given in the specification and the exogenous nucleic acid sequence encodes the peroxidase in a sequence that is codon-optimized for the host.
Filed: 4/2/2020
Application Number: US16838340A
Tech ID: SD 14164.1
This invention was made with Government support under Contract No. DE-NA0003525 awarded by the United States Department of Energy/National Nuclear Security Administration. The Government has certain rights in the invention.
Data from Derwent World Patents Index, provided by Clarivate
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