Real-time patch-clamp sensing of transport across a barrier tissue

DWPI Title: System for measuring electrical signals from cultured cell layer, such electrical signals are indication of transport or lack of transport of agent through cell layer, comprises first chamber, cultured layer disposed within first chamber, where cultured layer comprises multiple barrier cells
Abstract: The present disclosure relates to methods, apparatuses, and systems to measure one or more electrical signals from a cultured cell layer. In some embodiments, such electrical signals can be indicative of transport or lack of transport of an agent through the cell layer.
Use: System for measuring electrical signals from cultured cell layer, such electrical signals are indication of transport or lack of transport of agent through cell layer.
Advantage: The system is configured to receive the corresponding digital real component signal and corresponding imaginary component signal and to transmit a determined membrane capacitance as an output.
Novelty: System comprises first chamber (203), cultured layer disposed within the first chamber, where the cultured layer comprises multiple barrier cells seeded and then maintained within the first chamber. The cultured layer comprises tight junctions and/or adherens junctions. The system further comprises a substrate (201) comprising one or more pores (202) disposed within the substrate, where one pore is configured to form a seal with one barrier cell (110), and a pore is a micropore or a nanopore. A second chamber (204) is set in fluidic communication with one pore. A probe configured to detect an electrical signal within a fluid disposed within the second chamber, and a circuit configured to receive the electrical signal as an input signal and to transmit a real component and an imaginary component of complex admittance as output signals.
Filed: 10/19/2020
Application Number: US17074221A
Tech ID: SD 15162.0
This invention was made with Government support under Contract No. DE-NA0003525 awarded by the United States Department of Energy/National Nuclear Security Administration. The Government has certain rights in the invention.
Data from Derwent World Patents Index, provided by Clarivate
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