Protein and nucleic acid detection for microfluidic devices
| DWPI Title: Detecting nucleic acid target and polypeptide target in fluid sample by introducing fluid sample to system, generating complex containing nucleic acid and complex containing polypeptide, and detecting signal from label agent |
| Abstract: The present invention relates to methods for detecting targets by employing a temperature control system with a microfluidic device. The system allows for non-contact heating by employing an infrared emitter. In some instances, the system can be used in conjunction with a centrifugal microfluidic device. Optionally, a mask can be implemented to provide selective heating of desired assay areas of the device. |
| Use: The method is useful for detecting nucleic acid target and polypeptide target in fluid sample (claimed). |
| Advantage: The system used in the method allows simultaneous detection on a single device e.g. centrifugal microfluidic disc. |
| Novelty: Method for detecting a nucleic acid target and a polypeptide target in a fluid sample involves (a) introducing fluid sample to a system comprising a microfluidic disc comprising a substrate, a first assay area and a second assay area, (b) generating several first complexes on several first particles in the first assay area, where at least one first complex comprises the nucleic acid target and a first label agent, (c) generating several second complexes containing polypeptide target on several second particles in the second assay area, (d) transporting several first particles through a first density medium in the first assay area and second particles through a second density medium in the second assay area, and (e) detecting a signal from the first label agent of the first complex bound to the first particle and/or the second label agent of the second complex bound to the second particle. |
| Filed: 4/30/2019 |
| Application Number: US16399484A |
| Tech ID: SD 14385.0 |
| This invention was made with Government support under Contract No. DE-NA0003525 awarded by the United States Department of Energy/National Nuclear Security Administration. The Government has certain rights in the invention. |
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