Production of fusel lactates via biocatalysis
| DWPI Title: Converting glucose-containing hydrolyzed biomass into fusel lactate, which is ethyl lactate, involves expressing esterase gene in bacterial host, maintaining condition for fermenting in hydrolyzed biomass to produce alcohol and lactate and catalyzing reaction through enzyme encoded by esterase gene |
| Abstract: Microbial enzymes are used for esterification of biomass-derived substrates for production of industrially valuable esters. E. coli was used as an efficient platform for recombinant synthesis of fusel lactates such as the green solvent ethyl lactate. |
| Use: Method for converting a glucose-containing hydrolyzed biomass into a fusel lactate, which is selected from ethyl lactate, isopentyl lactate, isopropyl lactate, (iso)butyl lactate, and (iso)pentyl lactate (claimed). |
| Advantage: The method uses the biosynthesis process directly from a hydrolyzed biomass to eliminate cost of glucose and fermenting process additives in the making of the fusel lactates. |
| Novelty: Converting a glucose-containing hydrolyzed biomass into a fusel lactate involves (a) expressing an esterase gene in a bacterial host, where bacterial host is added to or already present in the glucose-containing hydrolyzed biomass; (b) maintaining conditions suitable for fermenting in the hydrolyzed biomass to produce an alcohol and a lactate; and (c) catalyzing reaction through an enzyme encoded by the esterase gene, alcohol and lactate to form a fusel lactate in the bacterial host, where enzyme is selected from ethyl-ester-synthase-1, acetylxylan-esterase-2, carbohydrate-esterase, esterase-A, truncated-esterase-A, and its combinations, and the esterase gene is obtained from an organism selected from Komagataella phaffii , Saccharomyces cerevisiae, Brettanomyces bruxellensis , or Pseudomonas aeruginosa . |
| Filed: 8/26/2020 |
| Application Number: US17003061A |
| Tech ID: SD 14908.0 |
| This invention was made with Government support under Contract No. DE-NA0003525 awarded by the United States Department of Energy/National Nuclear Security Administration. The Government has certain rights in the invention. |
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