Modified yeast microorganisms to increase yield of 3-hydropropionic acid
| DWPI Title: New engineered Basidiomycete organism has deletion of gene comprising certain sequence identity to nucleotide sequence in its genome, where Basidiomycete organism is selected from Rhodosporidium toruloides, Rhodosporidium azoricum, Rhodosporidium fluviale, for producing 3 hydroxypropionic acid |
| Abstract: Methods and compositions (e.g., engineered hosts) are disclosed for use in converting biomass to 3-hydropropionic acid. In particular embodiments, the methods include use of an engineered Rhodosporidium yeast, such as R. toruloides, the engineered R. toruloides having the RT04_8975 gene deleted from its genome, combined with a lignocellulosic hydrolysate, sourced, for example, from a biomass. A promoter for enhancing transport of 3HP is also incorporated by addition to the R. toruloides genome, for example, by modified lithium acetate transformation. |
| Use: Engineered Basidiomycete organism for use in preparing fermentation broth composition, and producing 3 hydroxypropionic acid (all claimed). |
| Advantage: The engineered Basidiomycete organism i.e. Rhodosporidium toruloides enables microbial production of 3 hydroxypropionic acid (3HP) from lignocellulosic hydrolysate at high titers, representing a significant step toward enabling industrial production of 3HP in the future. |
| Novelty: Engineered Basidiomycete organism is new, where engineered Basidiomycete organism comprises a deletion of a gene comprising at least 90% sequence identity to the nucleotide sequence of 545 amino acids (SEQ ID NO: 1), given in the specification in its genome, where the Basidiomycete organism is selected from Rhodosporidium toruloides, Rhodosporidium azoricum, Rhodosporidium fluviale, Rhodosporidium lusitaniae, Rhodosporidium babievae, Rhodosporidium diobovatum, Rhodosporidium paludigenum, Rhodosporidium sphaerocarpum, and Rhodosporidium kratochvilovae. |
| Filed: 3/15/2023 |
| Application Number: US18121697A |
| Tech ID: SD 16353.0 |
| This invention was made with Government support under Contract No. DE-NA0003525 awarded by the United States Department of Energy/National Nuclear Security Administration. The Government has certain rights in the invention. |
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