Miniature acoustic wave lysis system and uses thereof
| DWPI Title: Miniature acoustic cell lysis system used for clinical point-of-care diagnostics for detection of infectious targets, comprises cartridge comprising many channels, platform comprising transducer array, and thermal exchange layer |
| Abstract: The present invention relates to an acoustic lysis system including a disposable cartridge that can be reversibly coupled to a platform having a small, high-frequency piezoelectric transducer array. In particular, the system releases viable DNA, RNA, and proteins from human or bacterial cells, without chemicals or additional processing, to enable high-speed sample preparation for clinical point-of-care medical diagnostics and use with nano/microfluidic cartridges. Also described herein are methods of making and using the system of the invention. |
| Use: The system is useful for clinical point-of-care diagnostics for the detection of infectious targets. |
| Advantage: The system: employs high-frequency acoustic waves that rapidly disrupt cellular membranes without chemical treatment, thus releasing the intracellular material without damage or undesirable physical or chemical modification; and is rapid, accurate, and inexpensive. |
| Novelty: Miniature acoustic cell lysis system (100) comprises: a cartridge (140) comprising many channels, where each channel is configured to receive at least one test sample; a platform (150) comprising a transducer array, which comprises many acoustic transducers (120); and a thermal exchange layer (130) configured to be disposed between the cartridge and the transducer array. The transducer array is configured to be coupled reversibly to the cartridge, where each acoustic transducer is configured to be disposed beneath each channel and is adapted to propagate an acoustic wave in the channel. |
| Filed: 6/27/2014 |
| Application Number: US14318364A |
| Tech ID: SD 12600.0 |
| This invention was made with Government support under Contract No. DE-NA0003525 awarded by the United States Department of Energy/National Nuclear Security Administration. The Government has certain rights in the invention. |
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