| Abstract: |   Embodiments of microfluidic hubs and systems are described that may be
     used to connect fluidic modules. A space between surfaces may be set by
     fixtures described herein. In some examples a fixture may set
     substrate-to-substrate spacing based on a distance between registration
     surfaces on which the respective substrates rest. Fluidic interfaces are
     described, including examples where fluid conduits (e.g. capillaries)
     extend into the fixture to the space between surfaces. Droplets of fluid
     may be introduced to and/or removed from microfluidic hubs described
     herein, and fluid actuators may be used to move droplets within the space
     between surfaces. Continuous flow modules may be integrated with the hubs
     in some examples. |