Methods of transfection using sonoporation

DWPI Title: Transforming a cell involves introducing cell and agent into channel of acoustic cell system, providing radiofrequency signal to acoustic transducer, and propagating acoustic wave and generating localized acoustic pressure in proximity to cell and delivering agent into cell to transform cell
Abstract: The present invention relates to methods of transforming a cell by use of an acoustic transducer. In particular embodiments, the radiofrequency signal to the transducer can be tuned to provide optimal membrane disruption of the cell; and an agent, such as a nucleic acid, can then be delivered to the cell.
Use: Method for transforming a cell.
Advantage: The method provides optimal membrane disruption of cell.
Novelty: Transforming a cell involves (a) introducing a cell and an agent into at least one channel of an acoustic cell system comprising an acoustic transducer, where acoustic transducer is configured to be disposed beneath at least one channel and is adapted to propagate an acoustic wave in the at least one channel; (b) providing a radiofrequency signal to the acoustic transducer, and propagating the acoustic wave and generating localized acoustic pressure in proximity to the cell; and (c) delivering the agent into the cell to transform the cell, where acoustic cell system comprises a surface acoustic wave system or a bulk acoustic wave system, and the cell comprises an algal cell, where introducing step is conducted in the presence of a nutrient medium, and the nutrient medium has a salinity of from 3-30%.
Filed: 6/20/2019
Application Number: US16446971A
Tech ID: SD 14531.1
This invention was made with Government support under Contract No. DE-NA0003525 awarded by the United States Department of Energy/National Nuclear Security Administration. The Government has certain rights in the invention.
Data from Derwent World Patents Index, provided by Clarivate
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