Method and apparatus for purifying nucleic acids and performing polymerase chain reaction assays using an immiscible fluid

DWPI Title: Conducting assay involves incubating nucleic acids, beads, and lysis buffer to form complexes, transporting complexes through immiscible liquid into density media, and thermocycling complexes, density media, and PCR components
Abstract: Embodiments of the present invention are directed toward devices, systems, and methods for purifying nucleic acids to conduct polymerase chain reaction (PCR) assays. In one example, a method includes generating complexes of silica beads and nucleic acids in a lysis buffer, transporting the complexes through an immiscible fluid to remove interfering compounds from the complexes, further transporting the complexes into a density medium containing components required for PCR where the nucleic acids disassociate from the silica beads, and thermocycling the contents of the density medium to achieve PCR. Signal may be detected from labeling agents in the components required for PCR.
Use: The method is useful for conducting an assay, and purifying nucleic acids and performing PCR assays using an immiscible fluid.
Novelty: Method of conducting an assay, involves incubating nucleic acids, beads, and a lysis buffer to form complexes, where individual one of the complexes comprises several nucleic acids and a bead, transporting the complexes through an immiscible liquid into a density media, where the density media contains PCR components, and thermocycling the complexes, density media, and PCR components to perform PCR on the nucleic acids in the density media.
Filed: 9/27/2017
Application Number: US15717524A
Tech ID: SD 12551.1
This invention was made with Government support under Contract No. DE-NA0003525 awarded by the United States Department of Energy/National Nuclear Security Administration. The Government has certain rights in the invention.
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