Linear peptides that mimic structural epitopes for antibody assays

DWPI Title: Identifying non-linear epitope involves mimicking one or more secondary structures in spatially adjacent discontiguous first and second subsequences of one or more protein by peptide, where first and second subsequences define peptide mimic
Abstract: A method including identifying one or more structures in adjacent sequences of one or more proteins that can be mimicked by a peptide; and assembling a peptide that mimics the one or more secondary structures. A method including assembling epitopes of linked pairs of discontiguous peptide subsequences that mimic one or more structures in sequences of a protein; and assaying antibodies against the epitopes.
Use: Method for identifying a non-linear epitope.
Novelty: Identifying a non-linear epitope involves mimicking one or more secondary structures in spatially adjacent discontiguous first and second subsequences of one or more proteins by a synthesized peptide, where the first and second subsequences define the peptide mimic include two to less than 10 amino acid residues, the secondary structures in the spatially adjacent first and second subsequences are separated by a distance of 10 angstroms or less, assembling a peptide that mimics the one or more secondary structures, where the secondary structures are stabilized using one covalent modification. The secondary structures are linked together as portion of a single synthesized peptide molecule by attaching peptide linkers to the C and N-termini of the first and second subsequences and cross-linking the first and second subsequences, where the assembled peptide has less than 20 amino acid residues.
Filed: 1/20/2014
Application Number: US14159308A
Tech ID: SD 12476.1
This invention was made with Government support under Contract No. DE-NA0003525 awarded by the United States Department of Energy/National Nuclear Security Administration. The Government has certain rights in the invention.
Data from Derwent World Patents Index, provided by Clarivate
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