Enzymatic cascade bioreactor
| DWPI Title: System useful for converting sucrose to beta-D-glucose for use as biofuel includes reservoir and series of porous packed columns comprising enzymes immobilized on inert media |
| Abstract: Disclosed is an apparatus and method for continuously converting sucrose to beta-D-glucose. The method comprises a three stage enzymatic reactor in which an aqueous solution of sucrose is first converted into a solution of fructose and alpha-D-glucose by passing it through a porous, packed column containing an inert media on which invertase is immobilized. This solution is then sent through a second packed column containing glucose isomerase and finally a third packed column containing mutarotase. Solution temperature and pH are adjusted to maximize glucose output. |
| Use: As a bio-fuel for use in a fuel cell to power a mechanical/electrical device. |
| Advantage: The bioreactor converts complex sugar to simple sugars by enzymatic cascade reaction through systematic continuum of reactions and discharging in the system output stream by adjusting the solution temperature and pH to maximum glucose output. |
| Novelty: A system for converting sucrose to β-D-glucose, includes a reservoir comprising a solution of sucrose, pump and three separate columns attached in series. The first porous packed column comprises invertase, a second porous packed column comprising glucose isomerase, a third porous packed column comprising mutarotase, and device for heating the three porous packed columns. |
| Filed: 3/14/2005 |
| Application Number: US200580901A |
| Tech ID: SD 8539.0 |
| This invention was made with Government support under Contract No. DE-NA0003525 awarded by the United States Department of Energy/National Nuclear Security Administration. The Government has certain rights in the invention. |
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