Enrichment of amino acids from biomass residuum
| DWPI Title: Providing biomass residuum involves pre-treating biomass with acids and/or enzymes by providing biocomponents, fermenting biocomponents with genetically engineered organism, degrading genetically engineered organism, and separating first fermentation product by providing mixed alcohol portion |
| Abstract: The present invention relates to methods of providing a biomass residuum and compositions thereof. In particular examples, the biomass residuum includes one or more high value amino acids, even after removal of mixed alcohol components. In particular, the methods include implementing pre-treatment conditions and employing fermentation conditions including modified organisms. |
| Use: Method for providing a biomass residuum. |
| Advantage: The method enables improving fusel alcohol yield in algal protein anaerobic fermentation, reducing consumption rate of proteins in the hydrolyzates, water solubility and corrosivity, minimizing processing costs, and increasing theoretical yields. |
| Novelty: Providing a biomass residuum involves pre-treating a biomass with acids and/or enzymes by providing biocomponents comprising carbohydrates, amino acids, proteins, and peptides. The biocomponents are fermented with a first genetically engineered organism and a second genetically engineered organism by providing a first fermentation product. The first genetically engineered organism degrades the carbohydrates and the second genetically engineered organism degrades the amino acids, proteins, and peptides. The first fermentation product is separated by providing a mixed alcohol portion and the biomass residuum comprising 50-85% amino acids on a dry weight basis. The first genetically engineered organisms comprises an exogenous acetolactate synthase or a nucleic acid encoding the exogenous acetolactate synthase. The second genetically engineered organism comprises a modified alcohol dehydrogenase having increased reactivity with nicotinamide adenine dinucleotide (NADH). |
| Filed: 4/18/2019 |
| Application Number: US16388385A |
| Tech ID: SD 14888.0 |
| This invention was made with Government support under Contract No. DE-NA0003525 awarded by the United States Department of Energy/National Nuclear Security Administration. The Government has certain rights in the invention. |
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