Devices, systems, and methods for microscale isoelectric fractionation

Patent Number: 9,005,417
Issued: 4/14/2015
Official Filing: View the Complete Patent
Abstract: Embodiments of the present invention provide devices, systems, and methods for microscale isoelectric fractionation. Analytes in a sample may be isolated according to their isoelectric point within a fractionation microchannel. A microfluidic device according to an embodiment of the invention includes a substrate at least partially defining a fractionation microchannel. The fractionation microchannel has at least one cross-sectional dimension equal to or less than 1 mm. A plurality of membranes of different pHs are disposed in the microchannel. Analytes having an isoelectric point between the pH of the membranes may be collected in a region of the fractionation channel between the first and second membranes through isoelectric fractionation.
Filed: 10/1/2008
Application Number: 12/243,817
Related Opportunity: Microscale Immune and Cell Analysis (MICA)
Rapid Automated Point-of-Care System (RapiDx)
Government Interests: STATEMENT OF GOVERNMENT INTEREST This invention was made with Government support under Contract No. DE-NA0003525 awarded by the United States Department of Energy/National Nuclear Security Administration. The Government has certain rights in the invention.