Devices, systems, and methods for microscale isoelectric fractionation
| Patent Number: | 9,409,357 |
| Issued: | 8/9/2016 |
| Official Filing: | View the Complete Patent |
| Abstract: | Embodiments of the present invention provide devices, systems, and methods for microscale isoelectric fractionation. Analytes in a sample may be isolated according to their isoelectric point within a fractionation microchannel. A microfluidic device according to an embodiment of the invention includes a substrate at least partially defining a fractionation microchannel. The fractionation microchannel has at least one cross-sectional dimension equal to or less than 1 mm. A plurality of membranes of different pHs are disposed in the microchannel. Analytes having an isoelectric point between the pH of the membranes may be collected in a region of the fractionation channel between the first and second membranes through isoelectric fractionation. |
| Filed: | 1/23/2013 |
| Application Number: | 13/747,587 |
| Related Opportunity: |
Microscale Immune and Cell Analysis (MICA) Rapid Automated Point-of-Care System (RapiDx) |
| Government Interests: | STATEMENT OF GOVERNMENT INTEREST This invention was made with Government support under Contract No. DE-NA0003525 awarded by the United States Department of Energy/National Nuclear Security Administration. The Government has certain rights in the invention. |