Devices, systems, and methods for conducting assays with improved sensitivity using sedimentation
| Patent Number: | 8,962,346 |
| Issued: | 2/24/2015 |
| Official Filing: | View the Complete Patent |
| Abstract: | Embodiments of the present invention are directed toward devices, systems, and method for conducting assays using sedimentation. In one example, a method includes layering a mixture on a density medium, subjecting sedimentation particles in the mixture to sedimentation forces to cause the sedimentation particles to move to a detection area through a density medium, and detecting a target analyte in a detection region of the sedimentation channel. In some examples, the sedimentation particles and labeling agent may have like charges to reduce non-specific binding of labeling agent and sedimentation particles. In some examples, the density medium is provided with a separation layer for stabilizing the assay during storage and operation. In some examples, the sedimentation channel may be provided with a generally flat sedimentation chamber for dispersing the particle pellet over a larger surface area. |
| Filed: | 3/16/2012 |
| Application Number: | 13/423,050 |
| Related Opportunity: |
ViralFx SpinDx™: Point-of-Care Diagnostics Using Centrifugal Microfluidics |
| Government Interests: | STATEMENT OF GOVERNMENT INTEREST This invention was made with Government support under Contract No. DE-NA0003525 awarded by the United States Department of Energy/National Nuclear Security Administration. The Government has certain rights in the invention. |