Detection of cholinesterase inhibition with microfluidic devices and systems thereof
| DWPI Title: Conducting cholinesterase inhibition assay, involves generating multiple beads in fluid sample, transporting beads including complexes through density medium in detection chamber, and detecting presence or absence of first signal from activated cholinesterase in detection chamber |
| Abstract: The present invention relates to methods of conducting cholinesterase inhibition assays. In one instance, the assays can be configured to determine the presence of inactivated and activated cholinesterases. Also described herein are microfluidic devices and systems for conducting such assays. |
| Use: Method for conducting a cholinesterase inhibition assay. |
| Advantage: The method utilizes a bead-based format allows for separation of the captured cholinesterases from other components within the sample, possibly increasing sensitivity of assay. |
| Novelty: Conducting a cholinesterase inhibition assay, involves generating multiple beads in a fluid sample, transporting the beads including the complexes through a density medium in a detection chamber, and detecting a presence or an absence of a first signal from the activated cholinesterase in the detection chamber, where each bead of the beads comprises a first population of multiple complexes comprising a capture agent and an activated cholinesterase and a second population of multiple complexes comprising a capture agent and an inactivated cholinesterase, such that each bead comprises both inactivated cholinesterase and activated cholinesterase, the density medium has a density that is less than a density of the beads and more than a density of the fluid sample, and the transporting occurs, at least in portion, by sedimentation, the first signal arises from reacting the activated cholinesterase with a first probe and an optional second probe. |
| Filed: 1/7/2021 |
| Application Number: US17143363A |
| Tech ID: SD 14276.1 |
| This invention was made with Government support under Contract No. DE-NA0003525 awarded by the United States Department of Energy/National Nuclear Security Administration. The Government has certain rights in the invention. |
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