Detection of active and latent infections with microfluidic devices and systems thereof
| DWPI Title: Detecting targets of interest in sample by neutralizing mixture (treated with dissociation agent and comprising sample comprising anti-Ag85 or anti-p24 antibody) with neutralization agent, and detecting signal from detection agents |
| Abstract: The present invention relates to methods of detecting one or more targets of interest in a sample. In one instance, the target can be correlated to an active infection (e.g., by a virus and/or a bacterium). Methods can include treating the sample with a dissociation agent, thereby releasing the target of interest for more accurate detection (e.g., by use of a sedimentation-based centrifugal microfluidic devices). Also described herein are microfluidic devices and systems for use with a dissociation agent. |
| Use: The method is useful for detecting targets of interest in a sample (claimed). |
| Advantage: The methods, devices, and systems can be adapted for single detection of a target or for multiplexed detection of targets, thus enhancing selectivity and/or sensitivity of the assay. The target of interest can form a complex within the sample, such that a dissociation agent can be used to disrupt that complex, which in turn enhances detection of the target. Further, the method relies on the use of a density medium to effectively isolate the target of interest, thus simplifying detection of the target. A fluorescence signal can be more easily detected across the narrowed region due to reduced scattering, thus increasing the sensitivity of the assay. |
| Novelty: Detecting targets of interest in a sample involves providing a mixture comprising a sample, the sample comprising an anti-Ag85 antibody or an anti-p24 antibody that binds to a 1st target of interest, the sample treated with a dissociation agent, where the dissociation agent comprises an acid or an acidic buffer and where the dissociation agent is configured to dissociate the anti-Ag85 antibody or the anti-p24 antibody from the 1st target of interest; neutralizing the mixture with a neutralization agent configured to neutralize the dissociation agent; layering the treated mixture on a density medium disposed within a detection chamber that is disposed within a substrate; subjecting the treated mixture to a sedimentation force such that the 1st population of beads, or a portion, travels through the density medium; and detecting a presence or absence of a signal from one or more detection agents bound directly or indirectly to the 1st population of beads, or a portion. |
| Filed: 10/17/2017 |
| Application Number: US15785708A |
| Tech ID: SD 13820.1 |
| This invention was made with Government support under Contract No. DE-NA0003525 awarded by the United States Department of Energy/National Nuclear Security Administration. The Government has certain rights in the invention. |
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