CRISPR-Cas based system for targeting single-stranded sequences
| DWPI Title: Conducting cleavage assay for targeting nucleic acid sequences, involves incubating synthetic guiding component with nuclease and single-stranded target sequence, and cleaving single-stranded target sequence without short DNA oligomer containing proto-spacer adjacent motif sequence |
| Abstract: The present invention relates to a CRISPR-Cas based system for targeting nucleic acid sequences. In part, the invention relates to synthetic guiding components for targeting single-stranded sequences, as well as design principles for constructing such components. Also described herein are methods of employing such components, e.g., to repress or activate a desired target within the subject. |
| Use: Method for conducting cleavage assay used for targeting nucleic acid sequences. |
| Advantage: The method enables to improve cleavage efficiency, reduces substrate-ribonucleoprotein (RNP) stability, and promotes Cas9 access to targeting site. |
| Novelty: Conducting cleavage assay involves incubating synthetic guiding component with nuclease and single-stranded target sequence; where synthetic guiding component includes targeting portion configured to bind and cleave single-stranded target sequence; and cleaving single-stranded target sequence without short DNA oligomer containing proto-spacer adjacent motif (PAM) sequence (PAMmer). |
| Filed: 12/13/2018 |
| Application Number: US16219779A |
| Tech ID: SD 14537.1 |
| This invention was made with Government support under Contract No. DE-NA0003525 awarded by the United States Department of Energy/National Nuclear Security Administration. The Government has certain rights in the invention. |
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