Portable dual field gradient force multichannel flow cytometer device with a dual wavelength low noise detection scheme
| DWPI Title: Micro fluidic particle manipulation apparatus of bench-top optical detector system, has magnet that applies magnetic force on magnetic particles focused into single file streams, simultaneously with dielectrophoretic force |
| Abstract: Systems and methods for combining dielectrophoresis, magnetic forces, and hydrodynamic forces to manipulate particles in channels formed on top of an electrode substrate are discussed. A magnet placed in contact under the electrode substrate while particles are flowing within the channel above the electrode substrate allows these three forces to be balanced when the system is in operation. An optical detection scheme using near-confocal microscopy for simultaneously detecting two wavelengths of light emitted from the flowing particles is also discussed. |
| Use: Micro fluidic particle manipulation apparatus of bench-top optical detector system. |
| Advantage: Since the magnet is placed in contact under the electrode substrate, the dielectrophoresis, magnetic forces, and hydrodynamic forces flown through the magnetic particles located above the substrate can be balanced effectively. Hence, the particles can be fractionated effectively based on size for sample preparation applications, and the magnetic particles can be pulled to the micro fluidic chip surface reliably. The wavelengths of light emitted through the fluorescence of target analyte bound to the particles can be detected simultaneously using near-confocal microscopy and customized software detection technologies. Large number of streams of single file particles are optically interrogated in the micro fluidic particle manipulation apparatus, thus the need of large volumes of liquid for sheath-flow focusing of particles is eliminated. |
| Novelty: The apparatus has several electrodes (508,510) that are applied with voltage to impart dielectrophoretic force on magnetic particles located above substrate. The voltage is tuned to focus magnetic particles into multiple single file streams. The electrodes are provided with bi-pronged interdigitated electrode array with converging and diverging electrode gaps (506,512). The magnetic force imparted by magnet arranged proximate to substrate on magnetic particles is applied simultaneously with dielectrophoretic force, so as to levitate magnetic particles at height above substrate. |
| Filed: 11/2/2009 |
| Application Number: US2009610409A |
| Tech ID: SD 10961.1 |
| This invention was made with Government support under Contract No. DE-NA0003525 awarded by the United States Department of Energy/National Nuclear Security Administration. The Government has certain rights in the invention. |
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