Microfluidic device for acoustic cell lysis

DWPI Title: Microfluidic device for e.g. lysis comprises channel formed in microfluidic substrate adapted to flow of fluid and acoustic transducer disposed on lysis portion of channel adapted to propagate acoustic wave and generate acoustic pressure
Abstract: A microfluidic acoustic-based cell lysing device that can be integrated with on-chip nucleic acid extraction. Using a bulk acoustic wave (BAW) transducer array, acoustic waves can be coupled into microfluidic cartridges resulting in the lysis of cells contained therein by localized acoustic pressure. Cellular materials can then be extracted from the lysed cells. For example, nucleic acids can be extracted from the lysate using silica-based sol-gel filled microchannels, nucleic acid binding magnetic beads, or Nafion-coated electrodes. Integration of cell lysis and nucleic acid extraction on-chip enables a small, portable system that allows for rapid analysis in the field.
Use: A microfluidic device for acoustic cell lysis.
Advantage: Power delivered to the device can be chosen to maximize lysing efficiency without causing cavitation or excessive heating of the transducer and heat sink.
Novelty: A microfluidic device (10) comprises channel (14) formed in microfluidic substrate (15) adapted to flow of fluid which consists of biological cells and ≥ 1 acoustic transducer (12) disposed on lysis portion of channel adapted to propagate acoustic wave in fluid and generate localized acoustic pressure in lysis portion to lyse biological cells in fluid by acoustic pressure in which operating frequency of ≥ 1 acoustic transducer is 10-100 MHz.
Filed: 8/31/2010
Application Number: US2010872919A
Tech ID: SD 11592.0
This invention was made with Government support under Contract No. DE-NA0003525 awarded by the United States Department of Energy/National Nuclear Security Administration. The Government has certain rights in the invention.
Data from Derwent World Patents Index, provided by Clarivate
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