System and method for detecting components of a mixture including a valving scheme for competition assays

DWPI Title: Conducting competition assay for detection of target analyte, comprises e.g. loading sample into first chamber of assay device, and incubating sample and beads to allow formation of complexes of beads and target analyte
Abstract: Examples are described including measurement systems for conducting competition assays. A first chamber of an assay device may be loaded with a sample containing a target antigen. The target antigen in the sample may be allowed to bind to antibody-coated beads in the first chamber. A control layer separating the first chamber from a second chamber may then be opened to allow a labeling agent loaded in a first portion of the second chamber to bind to any unoccupied sites on the antibodies. A centrifugal force may then be applied to transport the beads through a density media to a detection region for measurement by a detection unit.
Use: The method is useful for conducting a competition assay for detection of a target analyte.
Advantage: The method performs competition assay quickly, accurately, and at a relatively low cost.
Novelty: Conducting a competition assay for detection of a target analyte, comprises e.g.: loading a sample into a first chamber of an assay device; incubating the sample and beads to allow formation of complexes of beads and the target analyte when present in the sample; opening at least a portion of a control layer dividing the first chamber from a second chamber to provide a fluidic connection between the first chamber and a first portion of the second chamber; and allowing the labeling agent to bind to unoccupied sites on the antibodies in the first portion of the second chamber.
Filed: 6/7/2017
Application Number: US15616740A
Tech ID: SD 12686.1
This invention was made with Government support under Contract No. DE-NA0003525 awarded by the United States Department of Energy/National Nuclear Security Administration. The Government has certain rights in the invention.
Data from Derwent World Patents Index, provided by Clarivate
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