Method and apparatus for purifying nucleic acids and performing polymerase chain reaction assays using an immiscible fluid
| DWPI Title: Apparatus useful for performing assay, preferably PCR and for purifying nucleic acids comprises first fluidic feature containing fluid sample, second fluidic feature adjacent to first fluidic feature, and third fluidic feature |
| Abstract: Embodiments of the present invention are directed toward devices, systems, and methods for purifying nucleic acids to conduct polymerase chain reaction (PCR) assays. In one example, a method includes generating complexes of silica beads and nucleic acids in a lysis buffer, transporting the complexes through an immiscible fluid to remove interfering compounds from the complexes, further transporting the complexes into a density medium containing components required for PCR where the nucleic acids disassociate from the silica beads, and thermocycling the contents of the density medium to achieve PCR. Signal may be detected from labeling agents in the components required for PCR. |
| Use: The apparatus is useful for performing an assay (claimed), preferably PCR and for purifying nucleic acids. |
| Novelty: Apparatus comprises (a) first fluidic feature, which contains a fluid sample, the fluid sample includes several complexes, individual ones of complexes comprises several nucleic acids and bead, (b) second fluidic feature adjacent to first fluidic feature, the second fluidic feature contains an immiscible fluid having a density greater than that of fluid sample but lower than complexes, and (c) third fluidic feature adjacent to second fluidic feature, which contains density medium, the density medium contains components necessary for PCR. |
| Filed: 11/26/2013 |
| Application Number: US14090040A |
| Tech ID: SD 12551.0 |
| This invention was made with Government support under Contract No. DE-NA0003525 awarded by the United States Department of Energy/National Nuclear Security Administration. The Government has certain rights in the invention. |
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