Methods of expanding bacteriophage host-range and bacteriophage produced by the methods
| DWPI Title: Forming bacteriophages with expanded host-range, used to treat infection, comprises e.g. determining selected phage strain that infects host bacterial strain and not infect target-host bacteria and culturing e.g. host bacterial strain |
| Abstract: A method of producing novel bacteriophages with expanded host-range and bacteriophages with expanded host ranges are disclosed. The method produces mutant phage strains which are infectious to a second host and can be more infectious to their natural host than in their natural state. The method includes repeatedly passaging a selected phage strain into bacterial cultures that contain varied ratios of its natural host bacterial strain with a bacterial strain that the phage of interest is unable to infect; the target-host. After each passage the resulting phage are purified and screened for activity against the target-host via double-overlay assays. When mutant phages that are shown to infect the target-host are discovered, they are further propagated in culture that contains only the target-host to produce a stock of the resulting mutant phage. |
| Use: The methods are useful for producing bacteriophages with expanded host-range (claimed), where the bacteriophages are useful for treating infections. No biological data given. |
| Novelty: Producing (M1) bacteriophages with expanded host-range, comprises: (a) determining a selected phage strain that infects a host bacterial strain and not infect a target-host bacterial strain, where the selected phage strain is Clostridium sporogenes B1 phage or B3 phage; (b) culturing the host bacterial strain and the target-host bacterial strain separately to obtain a host culture and a target-host culture, respectively; (c) mixing the host culture and the target-host culture into various co-culture ratios of each, thus obtaining a series of many first co-cultures; (d) adding the selected phage strain to each of first co-cultures; (e) adding a predetermined mutagen to each of the first co-cultures; (f) incubating under bacterial culture conditions; (g) harvesting a resulting phage from each first co-cultures and purifying; and (h) applying resulting phage to a subsequent set of co-cultures identical to first co-cultures but naive to the phage. |
| Filed: 10/14/2015 |
| Application Number: US14883366A |
| Tech ID: SD 13347.1 |
| This invention was made with Government support under Contract No. DE-NA0003525 awarded by the United States Department of Energy/National Nuclear Security Administration. The Government has certain rights in the invention. |
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