Trajectory-based triggering system for hyperspectral imaging flow cytometer
| DWPI Title: Hyperspectral imaging flow cytometer for use in biological research, has analyzer for real-time multivariate analysis of acquired hyperspectral image of particle to direct microfluidic system to sort particle into bin |
| Abstract: A hyperspectral imaging flow cytometer can acquire high-resolution hyperspectral images of particles, such as biological cells, flowing through a microfluidic system. A trajectory-based triggering system can be used that will only trigger the acquisition of a hyperspectral image when an appropriate particle or cell is crossing an imaging line, thereby saving valuable resources and time. The hyperspectral imaging flow cytometer can provide detailed spatial maps of multiple emitting species, cell morphology information, and state of health. An optimized system can image about 20 cells per second. The hyperspectral imaging flow cytometer enables many thousands of cells to be characterized in a single session. |
| Use: Hyperspectral imaging flow cytometer for use in biological research. |
| Advantage: Maximizes the efficiency of the algorithms to provide real-time classification of cells and rapid sorting capabilities by using multiple processors on the personal computer to implement parallel processing. |
| Novelty: The hyperspectral imaging flow cytometer has microfluidic flow system for injecting sample of fluorescent particles into channel, and hyperspectral confocal imaging system having focal plane downstream from particle detection system for laterally scanning focused laser beam along imaging line at third time with laser scanning centered on predicted lateral location and acquiring hyperspectral image of fluorescence. An analyzer is for real-time multivariate analysis of acquired hyperspectral image of particle to direct microfluidic system to sort particle into bin. |
| Filed: 8/21/2017 |
| Application Number: US15681824A |
| Tech ID: SD 14417.0 |
| This invention was made with Government support under Contract No. DE-NA0003525 awarded by the United States Department of Energy/National Nuclear Security Administration. The Government has certain rights in the invention. |
| Data from Derwent World Patents Index, provided by Clarivate All rights reserved. Republication or redistribution of Clarivate content, including by framing or similar means, is prohibited without the prior written consent of Clarivate. Clarivate and its logo, as well as all other trademarks used herein are trademarks of their respective owners and used under license. |