Microfluidic platform for multiplexed detection in single cells and methods thereof
| DWPI Title: Performing multiplexed, single-cell analysis in microfluidic device, comprises e.g. incubating held cell with protein label, incubating resultant with the label, detaching resultant, and performing on-chip flow cytometry assay of resultant |
| Abstract: The present invention relates to a microfluidic device and platform configured to conduct multiplexed analysis within the device. In particular, the device allows multiple targets to be detected on a single-cell level. Also provided are methods of performing multiplexed analyses to detect one or more target nucleic acids, proteins, and post-translational modifications. |
| Use: The method is useful for performing multiplexed, single-cell analysis in microfluidic device for the target protein and/or the target nucleic acid, where the target protein and/or the target nucleic acid comprise microRNA, mRNA, DNA and/or proteins e.g. including post-translation modified proteins in single cells. |
| Advantage: The method allows multiple targets to be detected on a single-cell while minimizing sample consumption and reducing complicated and manual fluid handling procedures. |
| Novelty: Performing multiplexed, single-cell analysis in microfluidic device, comprises: (i) loading cell sample into device; (ii) holding portion of cell sample within each holding chamber; (iii) incubating resulting held cell with first protein label in each holding chamber; (iv) incubating resulting labeled cell with first nucleic acid label, thus providing multiplexed-labeled cell; (v) detaching multiplexed-labeled cell, thus providing detached, intact cell; and (vi) performing on-chip flow cytometry assay of detached, intact cell, thus performing multiplexed, single-cell analysis. |
| Filed: 12/18/2014 |
| Application Number: US14575886A |
| Tech ID: SD 12975.1 |
| This invention was made with Government support under Contract No. DE-NA0003525 awarded by the United States Department of Energy/National Nuclear Security Administration. The Government has certain rights in the invention. |
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