Endpoint detection of amplified nucleic acids

DWPI Title: Reaction mixture used for detecting presence of target nucleic acid in sample, comprises target nucleic acid, first amplicon, duplex comprising forward inner primer, backward inner primer, pair of outer primers, and pair of loop primers
Abstract: The present invention relates to probes and primers beneficial for conducting amplification assays, such as those including loop-mediated isothermal amplification reactions. Also described herein are methods for detecting targets using such probes and/or primers.
Use: The mixture is useful for detecting a presence of a target nucleic acid in a sample.
Novelty: Reaction mixture comprises: (i) target nucleic acid comprising regions, including a B3 region, B2 region, B1 region, F1c region, F2c region and F3c region from 5' to 3' direction; (ii) first amplicon having corresponding regions sufficiently complementary to regions in target nucleic acid; (iii) duplex comprising forward inner primer comprising first nucleic acid sequence and further comprising second nucleic acid sequence and quench probe comprising third nucleic acid sequence having sufficient complementarity to first portion of forward inner primer and further comprising quencher label operably linked to third nucleic acid sequence; (iv) backward inner primer comprising fourth nucleic acid sequence having sufficient complementarity to B2c region of first amplicon; (v) pair of outer primers comprising forward primer comprising sixth nucleic acid sequence having F3 region and backward primer comprising seventh nucleic acid having B3 region; and (vi) pair of loop primers.
Filed: 1/27/2016
Application Number: US15008285A
Tech ID: SD 13252.2
This invention was made with Government support under Contract No. DE-NA0003525 awarded by the United States Department of Energy/National Nuclear Security Administration. The Government has certain rights in the invention.
Data from Derwent World Patents Index, provided by Clarivate
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