Endpoint detection of amplified nucleic acids

DWPI Title: Detecting presence of target nucleic acid in sample, comprises combining sample with first primer, quench probe and polymerase, amplifying target nucleic acid sequence by incubating at and promoting hybridization of quench probe
Abstract: The present invention relates to probes and primers beneficial for conducting amplification assays, such as those including loop-mediated isothermal amplification reactions. Also described herein are methods for detecting targets using such probes and/or primers.
Use: The method is useful for detecting presence of target nucleic acid in sample.
Novelty: Detecting presence of target nucleic acid in sample, comprises combining the sample with first primer, quench probe and polymerase to provide reaction mixture, where the first primer comprises a first nucleic acid sequence having complementarity to site in the target nucleic acid and the quench probe comprises second nucleic acid sequence having sufficient complementarity to a first portion of the first primer and a quencher label operably linked to the second nucleic acid sequence, amplifying the target nucleic acid sequence, if present, in the reaction mixture by incubating at temperature T1 and promoting hybridization of the quench probe to the first primer by cooling to a temperature T3, where T3 is less than T1, thus providing discriminated endpoint signal indicative of presence or absence of the target nucleic acid.
Filed: 6/16/2020
Application Number: US16903075A
Tech ID: SD 13252.3
This invention was made with Government support under Contract No. DE-NA0003525 awarded by the United States Department of Energy/National Nuclear Security Administration. The Government has certain rights in the invention.
Data from Derwent World Patents Index, provided by Clarivate
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