Engineering RuBisCo for food safety

DWPI Title: New biological material comprising amino acid sequence has specified is used for engineering ribulose-1,5-bisphosphate carboxylase/oxygenase from algal biomass
Abstract: A method of improving a ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCo) to have a higher protein score is disclosed. The method includes the steps of: making a modified RbcL of the RuBisCo, by, on an RbcL unit of the RuBisCo, either substituting Met for Leu, Phe, Val, or Ile or combinations thereof; substituting Lys for Arg, Thr, or His or combinations thereof; or both of these substitutions. The modified RbcL consequently modifies the RuBisCo and is added to a biomass host where it is stable for homologous recombination. Plastid and nucleus integration was observed. Example RbcL sequences are disclosed with the desirable substitutions. The improved RuBisCo can be used as an improved proteinaceous food source for humans and animals.
Use: The material is useful for: engineering Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCo) from algal biomass.
Advantage: The material provides high quality digestible protein feedstocks that supplement existing agriculture.
Novelty: Biological material comprising amino acid sequence having at least 84% sequence identity to SEQ ID NOS: 2-5 (each comprising a fully defined sequence of 40 amino acids as given in the specification), where amino acid sequence is modified large subunit of the ribulose-1,5-bisphosphate carboxylase/oxygenase complex (RbcL) unit incorporated in modified ribulose-1,5-bisphosphate carboxylase/oxygenase, is new.
Filed: 9/30/2020
Application Number: US17038513A
Tech ID: SD 15512.0
This invention was made with Government support under Contract No. DE-NA0003525 awarded by the United States Department of Energy/National Nuclear Security Administration. The Government has certain rights in the invention.
Data from Derwent World Patents Index, provided by Clarivate
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