The µChemLab™: Bio Detector

Technology Summary

Sandia National Laboratories has developed a compact, hand-held detection system that places the capability of a fully functional chemistry laboratory at the fingertips of a trained field operator. Sandia's chemical analysis system can rapidly detect and analyze toxic agents such as bacteria, viruses, and protozoa.

The µChemLabTM was designed, demonstrated, and tested by Sandia researchers over several years. The technology was initially developed for homeland security, defense applications, war fighters, and first responders. A variety of applications and near-term commercialization opportunities now exist in markets such as air and water quality, medical diagnostics, biotechnology, and industrial process control.

Description

The µChemLabTM Bio Detector is a portable device for determining the presence of a target analyte and enabling fast microfluidic separations of biological samples with high sensitivity. The device miniaturizes bench-scale analyses using fabricated microchannels in a hand-held, low-power device. Many different separations can be run simultaneously, and identification of the compound of interest is determined from unique retention-time signatures.

Designed for the rapid detection of proteins, the µChemLabTM has been used to identify biotoxins such as ricin, staphylococcal enterotoxin B, and botulinum toxin; its capability has been extended in combination with innovative, automated sample preparation technology to enable the identification of viruses and bacteria. Parallel analysis channels provide highly accurate detection at nanomolar sensitivites with a low false alarm probability.

Publications

Julia A. Fruetel and Victoria A. VanderNoot. (2011). "CGE for Virus Identification." Methods in Molecular Biology.

Victoria A. VanderNoot, Ronald F. Renzi, Bruce P. Mosier, James L. Van de Vreugde, Isaac Shokair, and Brent L. Haroldsen. (2010). "Development of an Integrated Microfluidic Instrument for Unattended Water Monitoring Applications." Electrophoresis, 31, pp. 2632-2640.

Julia A. Fruetel, Jason A.A. West, Bert J. Debusschere, Kyle Hukari, Todd W. Lane, Habib N. Najm, Jose Ortega, Ronald F. Renzi, Isaac Shokair, and Victoria A. VanderNoot. (2008). "Rapid Identification of Viruses using Microfluidic Protein Profiling and Bayesian Classification." Analytical Chemistry, 80, pp. 9005-9012.

Julia Fruetel, Jason West, Bert Debusschere, Kyle Hukari, Todd Lane, Habib Najm, Jose Ortega, Ronald Renzi, Isaac Shokair and Victoria VanderNoot. (2008). "Identification of Viruses Using Microfluidic Protein Profiling and Bayesian classification." Analytical Chemistry. 80, pp 9005, 9012. [online]. Available: http://pubs.acs.org/doi/pdfplus/10.1021/ac801342m

Victoria VanderNoot, Brent Haroldsen, Wayne Einfeld, Yolanda Fintschenko. (2008). "Unattended Water Sensor for Detection of Toxins in Water Systems." Environmental & Water Resources Institute Currents. 9, pp 6-7. [online]. Available: http://email.asce.org/ewri/UnattendedWaterSensor.html

Shelly Pizzaro, Pamela Lane, Todd Lane, Evelyn Cruz, Brent Haroldsen, Victoria VanderNoot. (2008, December). "Bacterial characterization Using Protein Profiling in a Microchip Separations Platform." Electrophoresis. 28(24), pp 4697-4704. [online]. Available: http://onlinelibrary.wiley.com/doi/10.1002/elps.200700005/pdf

Jeanne Stachowiak, Erin Shugard, Bruce Mosier, Ronald Renzi, Pamela Caton, Scott Ferko, James Van de Vreugde, Daniel Yee, Brent Haroldsen, Victoria VanderNoot. (2007, June). "Autonomous Microfluidic Sample Preparation System for Protein Profile-Based Detection of Aerosolized Bacterial Cells and Spores." Analytical Chemistry. 79 (15), pp. 5763- 5770. [online]. Available: http://pubs.acs.org/doi/pdfplus/10.1021/ac070567z

Julia Fruetel, Ronald Renzi, Victoria VanderNoot, James Stamps, Brent Horn, Jay A. West, Scott Ferko, Robert Crocker, Christopher Bailey, Don Arnold, Boyd Wiedenman, Wen-Yee Choi, Daniel Yee, Isaac Shokair, Ernest Hasselbrink, Philip Paul, David Rakestraw, Debbie Padgen. (2005, March). "Microchip Separations of Protein Biotoxins Using an Integrated Hand-Held Device." Electrophoresis. 26(6), pp 1144-1154. [online]. Available: http://onlinelibrary.wiley.com/doi/10.1002/elps.200406194/pdf

Ronald Renzi, James Stamps, Brent Horn, Scott Ferko, Victoria VanderNoot, Jay A. West, Robert Crocker, Boyd Wiedenman, Daniel Yee, Julia Fruetel. (2005). "Hand-Held Microanalytical Instrument for Chip-Based Electrophoretic Separations of Proteins." Analytical Chemistry. 77(2), pp 435-441. [online]. Available: http://pubs.acs.org/doi/pdfplus/10.1021/ac049214f

Julia Fruetel, Brent Horn, Jay A. West, James Stamps, Victoria VanderNoot, Mary Clare Stoddard, Ronald Renzi, Debbie Padgen. (2002). "Engineered improvement of the generation-2 µChemLab biotoxin detector." µTAS 2002. Y Babu et al. (eds.) Kluwer Academic: Dordrecht. pp. 703-705.

Ronald Renzi, James Stamps, Robert Crocker, Boyd Wiedenman, Scott Ferko, Brent Horn, Daniel Yee, Victoria VanderNoot, Jay West, Julia Fruetel, Yolanda Fintschenko, Mary Clare Stoddard. (2002). "Engineered improvement of the generation-2 µChemLab biotoxin detector." µTAS 2002. Y Babu et al. (eds.) Kluwer Academic: Dordrecht. pp. 703-705.

Julia Fruetel, Ronald Renzi, Robert Crocker, Victoria VanderNoot, James Stamps, Daniel Yee, David Rakestraw, Philip Paul, Don Arnold, Christopher Bailey. (2001). "µChemLab/CB: Parallel Channel Analysis in an Integrated Microchip Device." 14th International Symposium on Microscale Separations and Analysis. Boston, MA, January 13-18.

Julia Fruetel, Robert Crocker, Victoria VanderNoot, Scott Ferko, James Stamps, Isaac Shokair, Daniel Yee, Ernest Hasselbrink. (2000). "Development of µChemlab/CB for detection of biotoxins." Proceedings of the First Joint Detection for Chemical and Biological Defense. pp. 131-139.

Conferences/Presentations

Julie Fruetel, Ron Renzi, Victoria VanderNoot, and Gabriela Chirica. "Meeting User Needs: Developing Field-Deployable Biodetection Systems Using a Microseparations Approach." 18th Annual Biodetection Technologies. June 23-24, 2011, Washington DC.

Victoria VanderNoot, Ron Renzi, Bruce Mosier, Isaac Shokair, James Van De Vreugde, Jerry Inman, and Brent Haroldsen. "The Unattended Water Sensor: a protein monitoring system for domestic water applications." 2010 Sustainable Water Management Conference & Exposition. April 11-13, 2010, Albuquerque, NM.

Victoria VanderNoot, Ron Renzi, Bruce Mosier, Isaac Shokair, James Van De Vreugde, Jerry Inman, and Brent Haroldsen. "The Unattended Water Sensor: a protein monitoring system for domestic water applications." October 5-6, 2009, Wetsus Water Congress, Leeuwarden Netherlands.

V. VanderNoot, E. Van Blarigan, D. Curtis, P. Lane, T. Lane. "HABLab: A microfabricated total analytical system (µtas) for detecting phytotoxins in water." 122nd AOAC Annual Meeting & Exposition. September 21-24, 2009, Dallas, TX.

Victoria A. VanderNoot, Julia A. Fruetel, Ronald F. Renzi, et. al. "Micro-Total Analytical Systems and other Field-capable Platforms: Challenges and Considerations." 28th Annual Meeting AOAC International Pacific Northwest Section. June 18-19, 2008, Tacoma, WA.

V. VanderNoot. "Portable bioagent detection systems for national security applications." Department of Homeland Security, 2007 Homeland Security Conference. August 21-24, 2007, Monterey, CA.

V. VanderNoot, D. Curtis, P. Lane, T. Lane. "Development of a portable and automated electrophoresis-based system for in-field toxin detection: HABLab." PacRim 2007, Pacific Rim Shellfish Conference. April 22-23, 2007, San Diego, CA.

V. VanderNoot, et al. "Integrated and automated sample preparation systems for aerosol and water monitoring applications." 2nd National Conference on Environmental Sampling and Detection for Bio-Threat Agents. October 25-27, 2006, New York, NY.

V. A. VanderNoot, G. S. Chirica, J. Cintron, J. A. West, J. Fruetel, T. W. Lane, P. F. Caton, K. Wally, B. L. Haroldsen. "Detection of bacterial biowarfare agents with a proteomics-based analyzer." 2nd Joint Conference on Point Detection for Chemical and Biological Defense. March 1-5, 2004, Williamsburg, VA.

V. VanderNoot, S. Pizarro, J. Fruetel, R. Renzi, J. Stamps. "Phosphoprotein signatures for early pathogen exposure detection using µChemLabTM." Mainstreaming Microfluidics. May 15-16, 2003, Boston, MA.

V. VanderNoot, B. Wiedenman, Y. Fintschenko, J. Fruetel. "Incorporation of sample preconcentration into the µChemLabTM/CB platform for enhanced sensitivity." 16th International Symposium on Microscale Separations and Analysis. January 17-22, 2003, San Diego, CA.

J. Fruetel, V. A. VanderNoot, B. Kirby, C. Hasselbrink, T. Shepodd. "Polymeric coating protocol for µChemLab/CB. 15th International Symposium on Microscale Separations and Analysis." April 13-18, 2002, Stockholm, Sweden.

V. A. VanderNoot, E. Keuhler, S. Pizarro. "Isoelectric focusing with laser-induced fluorescence detection of phosphoprotein signatures. 15th International Symposium on Microscale Separations and Analysis." April 13-18, 2002, Stockholm, Sweden.

Benefits

  • Hand-portable
  • Low power consumption
  • Rapid analyses (5 to 10 minutes)
  • Highly accurate detection at nanomolar sensitivities
  • Low false alarm rate

Applications and Industries

  • Identification of biotoxins such as ricin, staphylococcal enterotoxin B, and botulinum toxin
  • Identification of viruses and bacteria
  • Air and water quality testing, medical diagnostics, biotechnology, and industrial process control
  • Full amenability to DNA-based separations for sizing or fragment detection
          
    The µChemLabTM was used for bioagent detection in the BioBriefcase program. For more information:
    http://www.sandia.gov/mission/homeland/chembio/development/detection/biobriefcase.html

Intellectual Property

Title
ID Number
Patent Number
Date
Portable apparatus for separating sample and detecting target analytes 8412.1 7,452,507 11/18/2008
Issued
Modular high voltage power supply for chemical analysis 8427.0 7,161,334 01/09/2007
Issued
Low power, scalable multichannel high voltage controller 8409.0 7,012,342 03/14/2006
Issued
Modular high voltage power supply for chemical analysis 8427.1 7,400,119 07/15/2008
Issued
Modular high voltage power supply for chemical analysis 8427.2 7,710,086 05/04/2010
Issued
Technology IDSD# 8412.1; SD# 8427; SD# 8409; SD# 8427.1; SD# 8427.2Development StagePrototypeAvailabilityAvailablePublished09/29/2011Last Updated01/23/2013