| Abstract: | A microfluidic acoustic-based cell lysing device that can be integrated
with on-chip nucleic acid extraction. Using a bulk acoustic wave (BAW)
transducer array, acoustic waves can be coupled into microfluidic
cartridges resulting in the lysis of cells contained therein by localized
acoustic pressure. Cellular materials can then be extracted from the
lysed cells. For example, nucleic acids can be extracted from the lysate
using silica-based sol-gel filled microchannels, nucleic acid binding
magnetic beads, or Nafion-coated electrodes. Integration of cell lysis
and nucleic acid extraction on-chip enables a small, portable system that
allows for rapid analysis in the field. |