|Abstract: ||Embodiments of the present invention provide methods, microfluidic
devices, and systems for the detection of an active target agent in a
fluid sample. A substrate molecule is used that contains a sequence which
may cleave in the presence of an active target agent. A SNAP25 sequence
is described, for example, that may be cleaved in the presence of
Botulinum Neurotoxin. The substrate molecule includes a reporter moiety.
The substrate molecule is exposed to the sample, and resulting reaction
products separated using electrophoretic separation. The elution time of
the reporter moiety may be utilized to identify the presence or absence
of the active target agent.