Methods, microfluidic devices, and systems for detection of an active enzymatic agent
| DWPI Title: Microfluidic device, useful for detecting active enzymatic agent e.g. botulinum neurotoxin, has substrate defining portion of channels, membrane to block substrate molecule having reporter moiety and sequence, and reagent loading channel |
| Abstract: Embodiments of the present invention provide methods, microfluidic devices, and systems for the detection of an active target agent in a fluid sample. A substrate molecule is used that contains a sequence which may cleave in the presence of an active target agent. A SNAP25 sequence is described, for example, that may be cleaved in the presence of Botulinum Neurotoxin. The substrate molecule includes a reporter moiety. The substrate molecule is exposed to the sample, and resulting reaction products separated using electrophoretic separation. The elution time of the reporter moiety may be utilized to identify the presence or absence of the active target agent. |
| Use: The microfluidic device is useful for detecting an active enzymatic agent including botulinum neurotoxin and tetanus neurotoxin (all claimed). |
| Advantage: The portable microfluidic device rapidly and economically detects the active enzymatic agent with high sensitivity and reduced usage of samples and reagents in a less labor intensive manner. |
| Novelty: The microfluidic device comprises a substrate defining a portion of a first channel, a second channel and a third channel coupled between the first and second channels, a first membrane positioned in the first channel and configured to block a substrate molecule including a reporter moiety and a sequence configured to be cleaved by the active enzymatic agent, a reagent loading channel coupled to the first channel, where the third channel is which is coupled to the first channel at a location between the reagent loading channel and the first membrane, and a second membrane. |
| Filed: 8/20/2009 |
| Application Number: US2009544982A |
| Tech ID: SD 11282.0 |
| This invention was made with Government support under Contract No. DE-NA0003525 awarded by the United States Department of Energy/National Nuclear Security Administration. The Government has certain rights in the invention. |
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