Hyperspectral imaging flow cytometer

DWPI Title: Hyperspectral imaging flow cytometer has analyzer for real-time multivariate analysis of acquired hyperspectral image of particle to direct microfluidic system to sort particle into bin based on acquired image of particle
Abstract: A hyperspectral imaging flow cytometer can acquire high-resolution hyperspectral images of particles, such as biological cells, flowing through a microfluidic system. The hyperspectral imaging flow cytometer can provide detailed spatial maps of multiple emitting species, cell morphology information, and state of health. An optimized system can image about 20 cells per second. The hyperspectral imaging flow cytometer enables many thousands of cells to be characterized in a single session.
Use: Hyperspectral imaging flow cytometer.
Advantage: Allows larger cell populations to be screened and sorted more quickly by characterizing a larger number of compounds, as well as an increasing the reliability and statistical significance of the measurements. Obtains the highest possible image quality from an optimized hyperspectral imaging flow cytometer.
Novelty: The hyperspectral imaging flow cytometer has microfluidic flow system (20) for focusing a sample of fluorescent particles in a channel, synchronizing the particles to flow single file through an imaging field in the channel, and sorting the particles into separate bins in response to an analysis of an acquired image of each particle. An analyzer is for real-time multivariate analysis (40) of the acquired hyperspectral image of the particle to direct the microfluidic system to sort the particle into a bin.
Filed: 2/18/2013
Application Number: US13769724A
Tech ID: SD 11899.1
This invention was made with Government support under Contract No. DE-NA0003525 awarded by the United States Department of Energy/National Nuclear Security Administration. The Government has certain rights in the invention.
Data from Derwent World Patents Index, provided by Clarivate
All rights reserved. Republication or redistribution of Clarivate content, including by framing or similar means, is prohibited without the prior written consent of Clarivate. Clarivate and its logo, as well as all other trademarks used herein are trademarks of their respective owners and used under license.