| Abstract: | We have developed an microelectroporation device that combines
microarrays of oligonucleotides, microfluidic channels, and
electroporation for cell transfection and high-throughput screening
applications (e.g. RNA interference screens). Microarrays allow the
deposition of thousands of different oligonucleotides in microscopic
spots. Microfluidic channels and microwells enable efficient loading of
cells into the device and prevent cross-contamination between different
oligonucleotides spots. Electroporation allows optimal transfection of
nucleic acids into cells (especially hard-to-transfect cells such as
primary cells) by minimizing cell death while maximizing transfection
efficiency. This invention has the advantage of a higher throughput and
lower cost, while preventing cross-contamination compared to conventional
screening technologies. Moreover, this device does not require bulky
robotic liquid handling equipment and is inherently safer given that it
is a closed system. |