| Abstract: | The various technologies presented herein relate to identifying whether
an individual has taken, and/or is under the influence of, a restricted
drug. A density separation technique is utilized, wherein a sample (e.g.,
blood, saliva, urine, etc.,) which may include an analyte is exposed to a
first plurality of beads having an analyte attached thereto, a second
plurality of beads having a metabolite-specific antibody attached
thereto, and a plurality of fluorophore-labelled analyte-specific
antibodies. After incubation, any analyte in the sample (e.g.,
delta-9-THC) is bound to the fluorophore-labelled analyte-specific
antibodies, any free fluorophore-labelled analyte-specific antibodies are
attached to the analyte of the first beads, and any metabolite in the
sample is bound to the second antibody. By applying centrifugal
separation, the first beads move to a region which undergoes irradiation.
If no fluorescence occurs, the sample includes the analyte; if
fluorescence occurs, the sample does not include the analyte. |