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Publications for Rapid Viral Detection and Diagnostics

Summary

Researchers at Sandia are developing a novel approach to viral detection focusing on a simplified workflow and low-cost portable instrumentation that could be controlled by a smartphone. Sandia’s previous research with Zika indicated that, using Sandia’s QUASR assay chemistry, viral RNA can be detected directly from clinical matrices with no sample preparation-- in other words, without RNA extraction, which is the most time and labor-intensive step in standard laboratory diagnostic protocols. We are applying this novel detection scheme for rapid, portable detection of SARS-CoV-2 of both clinical and environmental samples.

Description

A smartphone-based diagnostic platform for rapid detection of Zika, chikungunya, and dengue viruses
Here we demonstrate highly sensitive and specific detection of Zika, chikungunya, and dengue viruses by coupling reverse-transcription loop-mediated isothermal amplification (RT-LAMP) with our recently developed quenching of unincorporated amplification signal reporters (QUASR) technique. We conduct reactions in a simple, inexpensive and portable “LAMP box” supplemented with a consumer class smartphone. The entire assembly can be powered by a 5V USB source such as a USB power bank or solar panel.

Priye, A., Bird, S., Light, Y. et al. A smartphone-based diagnostic platform for rapid detection of Zika, chikungunya, and dengue viruses. Sci Rep 7, 44778 (2017).

Quenching of Unincorporated Amplification Signal Reporters in Reverse-Transcription Loop-Mediated Isothermal Amplification Enabling Bright, Single-Step, Closed-Tube, and Multiplexed Detection of RNA Viruses
A simple technique that allows closed-tube, target-specific detection, based on inclusion of a dye-labeled primer that is incorporated into a target-specific amplicon if the target is present. A short, complementary quencher hybridizes to unincorporated primer upon cooling down at the end of the reaction, thereby quenching fluorescence of any unincorporated primer. 

Ball, C.S., Light, Y.K., Koh, C, Wheeler, S., Coffey, L., and Meagher, R. Quenching of Unincorporated Amplification Signal Reporters in Reverse-Transcription Loop-Mediated Isothermal Amplification Enabling Bright, Single-Step, Closed-Tube, and Multiplexed Detection of RNA Viruses. N. p., 2016.

Additional Information

Related technologies are available for licensing:  
QUASR      
SmartLAMP

SAND2020-7652 W
AvailabilityAvailablePublished07/28/2020Last Updated07/28/2020