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Method and apparatus for purifying nucleic acids and performing polymerase chain reaction assays using an immiscible fluid

United States Patent

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10,590,477
March 17, 2020
View the Complete Patent at the US Patent & Trademark Office
Embodiments of the present invention are directed toward devices, systems, and methods for purifying nucleic acids to conduct polymerase chain reaction (PCR) assays. In one example, a method includes generating complexes of silica beads and nucleic acids in a lysis buffer, transporting the complexes through an immiscible fluid to remove interfering compounds from the complexes, further transporting the complexes into a density medium containing components required for PCR where the nucleic acids disassociate from the silica beads, and thermocycling the contents of the density medium to achieve PCR. Signal may be detected from labeling agents in the components required for PCR.
15/717,524
September 27, 2017
1/1;
C12Q 1/68 (20180101); C12Q 1/686 (20180101); B01L 3/00 (20060101); B01L 7/00 (20060101);
STATEMENT REGARDING RESEARCH & DEVELOPMENT This invention was made with Government support under Contract No. DE-NA0003525 awarded by the United States Department of Energy/National Nuclear Security Administration. The Government has certain rights in the invention.