| Abstract: | A hyperspectral imaging flow cytometer can acquire high-resolution
hyperspectral images of particles, such as biological cells, flowing
through a microfluidic system. A trajectory-based triggering system can
be used that will only trigger the acquisition of a hyperspectral image
when an appropriate particle or cell is crossing an imaging line, thereby
saving valuable resources and time. The hyperspectral imaging flow
cytometer can provide detailed spatial maps of multiple emitting species,
cell morphology information, and state of health. An optimized system can
image about 20 cells per second. The hyperspectral imaging flow cytometer
enables many thousands of cells to be characterized in a single session. |