Methods of expanding bacteriophage host-range and bacteriophage produced by the methods

United States Patent

9,988,608
June 5, 2018
View the Complete Patent at the US Patent & Trademark Office
Bacteriophage Host-Range Expansion
A method of producing novel bacteriophages with expanded host-range and bacteriophages with expanded host ranges are disclosed. The method produces mutant phage strains which are infectious to a second host and can be more infectious to their natural host than in their natural state. The method includes repeatedly passaging a selected phage strain into bacterial cultures that contain varied ratios of its natural host bacterial strain with a bacterial strain that the phage of interest is unable to infect; the target-host. After each passage the resulting phage are purified and screened for activity against the target-host via double-overlay assays. When mutant phages that are shown to infect the target-host are discovered, they are further propagated in culture that contains only the target-host to produce a stock of the resulting mutant phage.
14/883,366
October 14, 2015
1/1;
C12N 7/00 (20060101); C12Q 1/02 (20060101);
STATEMENT CONCERNING FEDERALLY SPONSORED RESEARCH This invention was developed under Contract DE-AC04-94AL85000 between Sandia Corporation and the United States Department of Energy. The U.S. Government has certain rights in this invention.