Microfluidic devices, systems, and methods for quantifying particles using centrifugal force

United States Patent

9,186,668
November 17, 2015
View the Complete Patent at the US Patent & Trademark Office
SpinDx™: Point-of-Care Diagnostics Using Centrifugal Microfluidics
Embodiments of the present invention are directed toward microfluidic systems, apparatus, and methods for measuring a quantity of cells in a fluid. Examples include a differential white blood cell measurement using a centrifugal microfluidic system. A method may include introducing a fluid sample containing a quantity of cells into a microfluidic channel defined in part by a substrate. The quantity of cells may be transported toward a detection region defined in part by the substrate, wherein the detection region contains a density media, and wherein the density media has a density lower than a density of the cells and higher than a density of the fluid sample. The substrate may be spun such that at least a portion of the quantity of cells are transported through the density media. Signals may be detected from label moieties affixed to the cells.
12/891,956
September 28, 2010
1/1;
C12Q 1/68 (20060101); B01L 3/02 (20060101); G01N 33/53 (20060101); B01L 3/00 (20060101); C12M 1/34 (20060101);
STATEMENT REGARDING RESEARCH & DEVELOPMENT Described examples were made with Government support under Government Contract No. DE-AC04-94AL85000 awarded by the U.S. Department of Energy to Sandia Corporation. The Government has certain rights in the invention, including a paid-up license and the right, in limited circumstances, to require the owner of any patent issuing in this invention to license others on reasonable terms.