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Methods and materials for deconstruction of biomass for biofuels production

United States Patent

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May 5, 2015
View the Complete Patent at the US Patent & Trademark Office
Trojan Horse Project: Biorefinery in a Plant
Next-Generation Automotive
The present invention relates to nucleic acids, peptides, vectors, cells, and plants useful in the production of biofuels. In certain embodiments, the invention relates to nucleic acid sequences and peptides from extremophile organisms, such as SSO1949 and Ce1A, that are useful for hydrolyzing plant cell wall materials. In further embodiments, the invention relates to modified versions of such sequences that have been optimized for production in one or both of monocot and dicot plants. In other embodiments, the invention provides for targeting peptide production or activity to a certain location within the cell or organism, such as the apoplast. In further embodiments, the invention relates to transformed cells or plants. In additional embodiments, the invention relates to methods of producing biofuel utilizing such nucleic acids, peptides, targeting sequences, vectors, cells, and/or plants.
March 30, 2012
800/288; 435/320.1; 536/23.1; 536/23.7; 800/278; 800/287; 800/295; 800/298;
C12N 15/82 (20060101); C12N 15/63 (20060101); C12N 15/09 (20060101); A01H 5/04 (20060101);
STATEMENT OF GOVERNMENT INTEREST This invention was made with Government support under Government Contract No. DE-AC04-94AL85000 awarded by the U.S. Department of Energy to Sandia Corporation. The Government has certain rights in the invention, including a paid-up license and the right, in limited circumstances, to require the owner of any patent issuing in this invention to license others on reasonable terms.