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Devices, systems, and methods for microscale isoelectric fractionation

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United States Patent

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9,005,417
April 14, 2015
View the Complete Patent at the US Patent & Trademark Office
Microscale Immune and Cell Analysis (MICA) Rapid Automated Point-of-Care System (RapiDx)
Embodiments of the present invention provide devices, systems, and methods for microscale isoelectric fractionation. Analytes in a sample may be isolated according to their isoelectric point within a fractionation microchannel. A microfluidic device according to an embodiment of the invention includes a substrate at least partially defining a fractionation microchannel. The fractionation microchannel has at least one cross-sectional dimension equal to or less than 1 mm. A plurality of membranes of different pHs are disposed in the microchannel. Analytes having an isoelectric point between the pH of the membranes may be collected in a region of the fractionation channel between the first and second membranes through isoelectric fractionation.
12/ 243,817
October 1, 2008
204/644204/548
B01D 57/02 (20060101)
;204/644,548,600,450;422/99,100,72,63,64,681;435/283.1,287.2,288.5,6;427/430
STATEMENT REGARDING RESEARCH & DEVELOPMENT The United States Government has a paid-up license in this invention and the right in limited circumstances to require the patent owner to license others on reasonable terms as provided for by the terms of contract No. DE-AC04-94AL85000 awarded by the U.S. Department of Energy to Sandia Corporation.