Devices, systems, and methods for conducting sandwich assays using sedimentation

United States Patent

February 3, 2015
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SpinDx™: Point-of-Care Diagnostics Using Centrifugal Microfluidics
Embodiments of the present invention are directed toward devices, systems, and method for conducting sandwich assays using sedimentation. In one example, a method includes generating complexes on a plurality of beads in a fluid sample, individual ones of the complexes comprising a capture agent, a target analyte, and a labeling agent. The plurality of beads including the complexes may be transported through a density media, wherein the density media has a density lower than a density of the beads and higher than a density of the fluid sample, and wherein the transporting occurs, at least in part, by sedimentation. Signal may be detected from the labeling agents of the complexes.
September 28, 2010
435/288.7; 427/2.11; 427/2.3; 435/286.5; 435/287.1; 435/288.6;
C12M 1/34 (20060101);
;435/288.7,286.5,287.1,288.6 ;427/2.11,2.3
STATEMENT REGARDING RESEARCH & DEVELOPMENT Described examples were made with Government support under Government Contract No. DE-AC04-94AL85000 awarded by the U.S. Department of Energy to Sandia Corporation. The Government has certain rights in the invention, including a paid-up license and the right, in limited circumstances, to require the owner of any patent issuing in this invention to license others on reasonable terms.