Devices, systems, and methods for conducting sandwich assays using sedimentation

United States Patent

8,945,914
February 3, 2015
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SpinDx™: Point-of-Care Diagnostics Using Centrifugal Microfluidics
Embodiments of the present invention are directed toward devices, systems, and method for conducting sandwich assays using sedimentation. In one example, a method includes generating complexes on a plurality of beads in a fluid sample, individual ones of the complexes comprising a capture agent, a target analyte, and a labeling agent. The plurality of beads including the complexes may be transported through a density media, wherein the density media has a density lower than a density of the beads and higher than a density of the fluid sample, and wherein the transporting occurs, at least in part, by sedimentation. Signal may be detected from the labeling agents of the complexes.
12/891,977
September 28, 2010
435/288.7; 427/2.11; 427/2.3; 435/286.5; 435/287.1; 435/288.6;
C12M 1/34 (20060101);
;435/288.7,286.5,287.1,288.6 ;427/2.11,2.3
STATEMENT REGARDING RESEARCH & DEVELOPMENT Described examples were made with Government support under Government Contract No. DE-AC04-94AL85000 awarded by the U.S. Department of Energy to Sandia Corporation. The Government has certain rights in the invention, including a paid-up license and the right, in limited circumstances, to require the owner of any patent issuing in this invention to license others on reasonable terms.