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Methods and devices for immobilization of single particles in a virtual channel in a hydrodynamic trap

United States Patent

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8,815,177
August 26, 2014
View the Complete Patent at the US Patent & Trademark Office
Microscale Immune and Cell Analysis (MICA)
Disclosed herein are methods of immobilizing a particle which comprise focusing the flow of a sample fluid containing the particle into a virtual channel which flows towards an unoccupied hydrodynamic trap in a microfluidic channel such that the particle flows into the hydrodynamic trap and becomes immobilized therein. Also disclosed are microfluidic devices which comprise at least one microchannel having at least one hydrodynamic trap, at least one focusing fluid inlet, said focusing fluid inlet is upstream of the hydrodynamic trap such that a focusing fluid introduced therein results in a virtual channel of a sample fluid when present which preferentially flows toward the hydrodynamic trap.
12/ 812,974
January 21, 2009
422/503 422/68.1
G01N 1/40 (20060101)B01L 3/00 (20060101)
Search:</B></TD><TDALIGN="RIGHT"VALIGN="TOP"WIDTH="70%">;422/68.1,500,501,502,503,504;436/174,177,183
ACKNOWLEDGEMENT OF GOVERNMENT SUPPORT Employees of Sandia National Laboratories made this invention. The government has certain rights in the invention.