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Method for concentration and separation of biological organisms by ultrafiltration and dielectrophoresis

United States Patent

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8,257,568
September 4, 2012
View the Complete Patent at the US Patent & Trademark Office
Insulating Dielectrophoresis (iDEP)
Disclosed is a method for monitoring sources of public water supply for a variety of pathogens by using a combination of ultrafiltration techniques together dielectrophoretic separation techniques. Because water-borne pathogens, whether present due to "natural" contamination or intentional introduction, would likely be present in drinking water at low concentrations when samples are collected for monitoring or outbreak investigations, an approach is needed to quickly and efficiently concentrate and separate particles such as viruses, bacteria, and parasites in large volumes of water (e.g., 100 L or more) while simultaneously reducing the sample volume to levels sufficient for detecting low concentrations of microbes (e.g., <10 mL). The technique is also designed to screen the separated microbes based on specific conductivity and size.
12/ 899,671
October 7, 2010
204/518 204/547
B01D 63/00 (20060101)G01N 27/26 (20060101)
204/518,547
STATEMENT OF GOVERNMENT SUPPORT This invention was made pursuant to Contract No. DE-AC04-94AL85000 by the United States Department of Energy and Sandia Corporation for the operation of Sandia National Laboratories and with funding from the Centers for Disease Control and Prevention, an agency of the United States Government. Therefore, the U.S. Government has certain rights in this invention.