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Insulator-based DEP with impedance measurements for analyte detection

United States Patent

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7,678,256
March 16, 2010
View the Complete Patent at the US Patent & Trademark Office
Insulating Dielectrophoresis (iDEP)
Disclosed herein are microfluidic devices for assaying at least one analyte specie in a sample comprising at least one analyte concentration area in a microchannel having insulating structures on or in at least one wall of the microchannel which provide a nonuniform electric field in the presence of an electric field provided by off-chip electrodes; and a pair of passivated sensing electrodes for impedance detection in a detection area. Also disclosed are assay methods and methods of making.
Davalos; Rafael V. (Blacksburg, VA), Simmons; Blake A. (San Francisco, CA), Crocker; Robert W. (Fremont, CA), Cummings; Eric B. (Livermore, CA)
Sandia Corporation (Livermore, CA)
11/556,685
November 3, 2006
204/547 ; 204/643;
G01N 27/447 (20060101); G01N 27/453 (20060101);
204/547,643
ACKNOWLEDGEMENT OF GOVERNMENT SUPPORT The United States Government has a paid-up license in this invention and the right in limited circumstances to recluire the patent owner to license to others on reasonable terms as provided for by the terms of contract DE-AC04-94AL85000 awarded by the U.S. Department of Energy to Sandia Corporation.