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Methods and devices for high-throughput dielectrophoretic concentration

United States Patent

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7,666,289
February 23, 2010
View the Complete Patent at the US Patent & Trademark Office
Insulating Dielectrophoresis (iDEP)
Disclosed herein are methods and devices for assaying and concentrating analytes in a fluid sample using dielectrophoresis. As disclosed, the methods and devices utilize substrates having a plurality of pores through which analytes can be selectively prevented from passing, or inhibited, on application of an appropriate electric field waveform. The pores of the substrate produce nonuniform electric field having local extrema located near the pores. These nonuniform fields drive dielectrophoresis, which produces the inhibition. Arrangements of electrodes and porous substrates support continuous, bulk, multi-dimensional, and staged selective concentration.
Simmons; Blake A. (San Francisco, CA)Cummings; Eric B. (Livermore, CA)Fiechtner; Gregory J. (Germantown, MD)Fintschenko; Yolanda (Livermore, CA)McGraw; Gregory J. (Ann Arbor, MI)Salmi; Allen (Escalon, CA)
Sandia Corporation (Livermore, CA)
12/ 202,489
September 2, 2008
204/643 204/600204/627210/321.75
B03C 5/02 (20060101)B01D 63/00 (20060101)G01N 27/00 (20060101)
204/547,515,451,643,600,627209/210422/50,68.1536/23.1210/321.6,321.75
ACKNOWLEDGEMENT OF GOVERNMENT SUPPORT This invention was made with Government support under government contract DE-AC04-94AL85000 awarded by the U.S. Department of Energy to Sandia Corporation. The Government has certain rights in the invention, including a paid-up license and the right, in limited circumstances, to require the owner of any patent issuing in this invention to license others on reasonable terms.